期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 104, 期 1, 页码 47-54出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2009.09.026
关键词
Lanthanide complexes; Contrast agents; Magnetic resonance imaging; P-glycoprotein; Blood-brain barrier
资金
- Universite Paris 13 [BQR/01/02/08]
- CNRS
- l'Association France Alzheimer, France
- Foundation of Science and Technology (F.C.T.), Portugal [PTDC/QUI/70063/2006]
- FEDER
Alzheimer's disease (AD) is the most commonly form of dementia in the elderly. The development of molecules able to detect biomarkers characteristic of AD is critical to its understanding and treatment. However, such molecules must be able to pass blood-brain barrier (BBB) which is a major impediment to the entry of many therapeutic drugs into the brain. Such a limitation applies to the development of magnetic resonance imaging molecular neuroimaging agents using biomarkers of AD-like P-amyloid deposits, as the common extracellular contrast agents (CAs) are not able to cross an intact BBB. In this work, we have studied the ability of a series of simple Eu3+ Complexes to enter cells overexpressing or not the ABCB1 (P-gp or P-glycoprotein) protein, which is expressed at the BBB and in human embryonic astrocytes. The intracellular uptake of the Eu3+ complexes of linear and macrocyclic polyaminocarboxylate ligands with different charges and lipophilicities was followed by atomic absorption spectrometry. Based on biochemical argument, we propose that lipophilic contrast agents can be efficiently taken up by cells and accumulate inside mitochondria when they are positively charged. The important point is that they are not P-gp substrates. which is one of the major obstacles for them to cross the BBB. (C) 2009 Elsevier Inc. All rights reserved.
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