4.4 Article

A Trypsin-Sensitive Proteoglycan from the Tapeworm Hymenolepis diminuta Inhibits Murine Neutrophil Chemotaxis in vitro by Suppressing p38 MAP Kinase Activation

期刊

JOURNAL OF INNATE IMMUNITY
卷 11, 期 2, 页码 136-149

出版社

KARGER
DOI: 10.1159/000492303

关键词

Chemotaxis; Neutrophils; Parasite-host interactions

资金

  1. Natural Science and Engineering Research Council of Canada (NSERC)
  2. NSERC CREATE Host-Parasite Interactions Program at University Calgary
  3. Canadian Institutes of Health Research (CIHR)/Canadian Association of Gastroenterology (CAG)/Allergan Inc.
  4. Alberta Innovates-Health solutions (AI-HS)

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It has emerged that neutrophils can play important roles in the host response following infection with helminth parasites. Mice infected with the tapeworm, Hymenolepis diminuta, are protected from some inflammatory conditions, accompanied by reduced neutrophil tissue infiltration. Thus, the ability of a phosphate-buffered saline-soluble extract of the worm (H. diminuta extract [HdE]) was tested for (1) its ability to activate murine neutrophils (Ca2+ mobilization, reactive oxygen species (ROS) and cytokine production); and (2) affect neutrophil chemotaxis in vitro to the penta-peptide, WKYMVm, the chemokine, KC, and leukotriene B-4. HdE was not cytotoxic to neutrophils, elicited a Ca(2+ )response and ROS, but not, cytokine (KC, interleukin-10, tumour necrosis factor-a) generation. HdE is not a chemotactic stimulus for murine neutrophils. However, a heat- and trypsin-sensitive, acid-insensitive proteoglycan (sensitive to sodium metaperiodate) in the HdE significantly reduced neutrophil chemotaxis towards WKYMVm or KC, but not LTB4 . The latter suggested that the HdE interfered with p38 mitogen-activated protein kinase signalling, which is important in WKYMVm chemotaxis. Corroborating this, immunoblotting revealed reduced phosphorylated p38, and the downstream signal heat-shock protein-27, in protein extracts from HdE + WkYMVm treated cells compared to those exposed to the penta-peptide only. We speculate that HdE can be used to modify the outcome of neutrophilic disease and that purification of the bioactive proteoglycan(s) from the extract could be used as a template to design immunomodulatory drugs targeting neutrophils. (C) 2018 The Author(s) Published by S. Karger AG, Basel

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