期刊
JOURNAL OF INNATE IMMUNITY
卷 6, 期 1, 页码 21-30出版社
KARGER
DOI: 10.1159/000351200
关键词
Staphylococcus aureus; USA300; SaeR/S; Two-component systems; Virulence; Gene expression; Neutrophils; alpha-Defensin
类别
资金
- National Institute of Health [NIH-RR020185, NIH-R01 award A1090046-01, NIH-R21 award R21 A1088041-01, R56-A1091856-01A1, NIH-A1083211]
- Molecular Biosciences Fellowship [P20RR16455-07]
- Montana State University Agriculture Experiment Station
- Murdoch Charitable Trust
- New York University School of Medicine Development Funds
- American Heart Association Scientist Development [09SDG2060036]
- NATIONAL CENTER FOR RESEARCH RESOURCES [P20RR020185, P20RR016455] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI090046, P01AI083211, R21AI088041, R56AI091856] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P30GM110732] Funding Source: NIH RePORTER
The ability of Staphylococcus aureus to infect tissues is dependent on precise control of virulence through gene-regulatory systems. While the SaeR/S two-component system has been shown to be a major regulator of S. aureus virulence, the influence of the host environment on SaeR/S-regulated genes (saeR/S targets) remains incompletely defined. Using QuantiGene 2.0 transcriptional assays, we examined expression of genes with the SaeR binding site in USA300 exposed to human and mouse neutrophils and host-derived peptides and during subcutaneous skin infection. We found that only some of the saeR/S targets, as opposed to the entire SaeR/S virulon, were activated within 5 and 10 min of interacting with human neutrophils as well as alpha-defensin. Furthermore, mouse neutrophils promoted transcription of saeR/S targets despite lacking alpha-defensin, and the murine skin environment elicited a distinctive expression profile of saeR/S targets.These findings indicate that saeR/S-mediated transcription is unique to and dependent on specific host stimuli. By using isogenic USA300 Delta saeR/S and USA300 Delta agr knockout strains, we also determined that SaeR/S is the major regulator of virulence factors, while Agr, a quorum-sensing two-component system, has moderate influence on transcription of the saeR/S targets under the tested physiological conditions. Copyright (C) 2013 S. Karger AG, Basel
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据