期刊
JOURNAL OF INNATE IMMUNITY
卷 5, 期 6, 页码 613-624出版社
KARGER
DOI: 10.1159/000350234
关键词
Allergy; Asthma; Flow cytometry; Host defense; Macrophages; Phagocytosis; gamma-Tocopherol
类别
资金
- National Center for Complementary and Alternative Medicine at the National Institutes of Health [P01AT002620]
- US Environmental Protection Agency [CR 83346301]
- United States Environmental Protection Agency [CR 83346301]
- Center for Environmental Medicine and Lung Biology at the University of North Carolina at Chapel Hill
Elevated inflammation and altered immune responses are features found in atopic asthmatic airways. Recent studies indicate gamma-tocopherol (GT) supplementation can suppress airway inflammation in allergic asthma. We studied the effects of in vitro GT supplementation on receptor-mediated phagocytosis and expression of cell surface molecules associated with innate and adaptive immunity on sputum-derived macrophages. Cells from nonsmoking healthy (n = 6) and mild house dust mite-sensitive allergic asthmatics (n = 6) were treated ex vivo with GT (300 mu M) or saline (control). Phagocytosis of opsonized zymosan A bioparticles (Saccharomyces cerevisiae) and expression of surface molecules associated with innate and adaptive immunity were assessed using flow cytometry. GT caused significantly decreased (p < 0.05) internalization of attached zymosan bioparticles and decreased (p < 0.05) macrophage expression of CD206, CD36 and CD86 in allergic asthmatics but not in controls. Overall, GT caused downregulation of both innate and adaptive immune response elements, and atopic status appears to be an important factor. Copyright (C) 2013 S. Karger AG, Basel
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