期刊
JOURNAL OF INNATE IMMUNITY
卷 5, 期 3, 页码 251-260出版社
KARGER
DOI: 10.1159/000345417
关键词
Bacteriology; Host defense; Proteinases
类别
资金
- Foundation for Polish Science (TEAM project) [DPS/424-329/10]
- National Science Centre, Poland [2011/01/D/NZ6/00269]
- Ministry of Science and Higher Education, Poland [IP2011 044371, IP2011 022171, 0095/B/P01/2009/37, 1642/B/P01/2008/35]
- Jagiellonian University [DS/9/WBBiB]
- National Institutes of Health, USA [DE 09761]
- European Union [POIG.02.01.00-12-064/08]
The pulmonary surfactant is a complex mixture of lipids and proteins that is important for respiratory lung functions, which also provides the first line of innate immune defense. Pulmonary surfactant protein-A (SP-A) is a major surfactant component with immune functions with importance during Staphylococcus aureus infections that has been demonstrated in numerous studies. The current study showed that S. aureus can efficiently cleave the SP-A protein using its arsenal of proteolytic enzymes. This degradation appears to be mediated by cysteine proteases, in particular staphopain A (ScpA). The staphopain-mediated proteolysis of SP-A resulted in a decrease or complete abolishment of SP-A biological activity, including the promotion of S. aureus phagocytosis by neutrophils, aggregation of Gram-negative bacteria and bacterial cell adherence to epithelium. Significantly, ScpA has also efficiently degraded SP-A in complete bronchi-alveolar lavage fluid from human lungs. This indicates that staphopain activity in the lungs is resistant to protease inhibitors, thus suggesting that SP-A can be cleaved in vivo. Collectively, this study showed that the S. aureus protease ScpA is an important virulence factor that may impair innate immunity of the lungs. Copyright (C) 2012 S. Karger AG, Basel
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据