期刊
JOURNAL OF INNATE IMMUNITY
卷 5, 期 3, 页码 242-250出版社
KARGER
DOI: 10.1159/000345356
关键词
CL-K1; Collectin-11; Complement activation; Lectin pathway; MAP-1; MASP-1; MASP-2; MASP-3
类别
资金
- Alfred Benzon Foundation
- Novo Nordisk Research Foundation
- Danish Cancer Society
- Svend Andersen Research Foundation
- Research Foundation of the Capital Region of Denmark
- Research Foundation of the Capital Region of Rigshospitalet
- Danish Medical Research Council
Collectins and ficolins are important in the clearance of endogenous and exogenous danger materials. A new human collectin-11 was recently identified in low concentration in serum in complex with mannose-binding lectin (MBL)/ficolin-associated serine proteases. Collectin-11 binds to carbohydrate residues present on various microorganisms. Thus, we hypothesized that collectin-11 could be a novel initiation molecule in the lectin pathway of complement. We can show that collectin-11 associates with all the known MBL-associated serine proteases (MASP-1, MASP-2 and MASP-3) as well as the lectin complement pathway regulator MAP-1. Furthermore, we found that complex formation between recombinant collectin-11 and recombinant MASP-2 on Candida albicans leads to deposition of C4b. Native collectin-11 in serum mediated complement activation and deposition of C4b and C3b, and formation of the terminal complement complex on C. albicans. Moreover, spiking collectin-11-depleted serum, which did not mediate complement activation, with recombinant collectin-11 restored the complement activation capability. These results define collectin-11 as the fifth recognition molecule in the lectin complement pathway in addition to MBL, ficolin-1, ficolin-2 and ficolin-3. Copyright (C) 2012 S. Karger AG, Basel
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