期刊
JOURNAL OF INFECTIOUS DISEASES
卷 210, 期 8, 页码 1188-1197出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiu236
关键词
Plasmodium falciparum; transmission dynamics; gametocytes; genotyping; capillary electrophoresis; pfg377; pfs230
资金
- Swiss National Science Foundation [310030_134889]
- International Centers of Excellence in Malaria Research [U19 AI089686]
- National Health and Medical Research Council [GNT1021544, GNT1043345]
- Bill and Melinda Gates Foundation [OPP1034577]
- Swiss National Science Foundation (SNF) [310030_134889] Funding Source: Swiss National Science Foundation (SNF)
- Bill and Melinda Gates Foundation [OPP1034577] Funding Source: Bill and Melinda Gates Foundation
Background. Differentiation between gametocyte-producing Plasmodium falciparum clones depends on both high levels of stage-specific transcripts and high genetic diversity of the selected genotyping marker obtained by a high-resolution typing method. By analyzing consecutive samples of one host, the contribution of each infecting clone to transmission and the dynamics of gametocyte production in multiclone infections can be studied. Methods. We have evaluated capillary electrophoresis based differentiation of 6 length-polymorphic gametocyte genes. RNA and DNA of 25 mu L whole blood from 46 individuals from Burkina Faso were simultaneously genotyped. Results. Highest discrimination power was achieved by pfs230 with 18 alleles, followed by pfg377 with 15 alleles. When assays were performed in parallel on RNA and DNA, 85.7% of all pfs230 samples and 59.5% of all pfg377 samples contained at least one matching genotype in DNA and RNA. Conclusions. The imperfect detection in both, DNA and RNA, was identified as major limitation for investigating transmission dynamics, owing primarily to the volume of blood processed and the incomplete representation of all clones in the sample tested. Abundant low-density gametocyte carriers impede clone detectability, which may be improved by analyzing larger volumes and detecting initially sequestered gametocyte clones in follow-up samples.
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