期刊
JOURNAL OF INFECTIOUS DISEASES
卷 207, 期 7, 页码 1128-1134出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jis939
关键词
Acinetobacter baumannii; complement; serum resistance; biofilm; innate immunity
资金
- University of Mississippi Medical Center
- University of Texas Health Science Center at Tyler
Acinetobacter baumannii is an important nosocomial pathogen. Infections are often preceded by intubation or catheter use, promoting the formation of biofilm, and some strains are able to cause severe cases of bacteremia because of their ability to resist killing by complement. We identified a secreted serine protease, termed PKF, that provided resistance to complement killing and suppressed biofilm formation. Serum resistance was abrogated in A. baumannii treated with protease inhibitors, as well as in a PKF-negative mutant. Serum resistance could be restored by recombinant PKF, which was shown to reduce the complement activity of normal human serum by almost 50%. PKF was shown to inhibit biofilm formation, because the PKF-negative mutant and wild-type A. baumannii treated with protease inhibitors produced biofilm that could be inhibited by addition of recombinant PKF. Our data indicate that PKF is required for serum resistance and that it suppresses biofilm formation in A. baumannii.
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