期刊
JOURNAL OF INFECTIOUS DISEASES
卷 202, 期 11, 页码 1738-1748出版社
OXFORD UNIV PRESS INC
DOI: 10.1086/656721
关键词
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资金
- National Health and Medical Research Council (NHMRC)
- Alfred Research Trust
- National Health Group Singapore
Background. Naive T cell recovery is critical for successful immune reconstitution after antiretroviral therapy (ART), but the relative contribution of CD31(+) and CD31(-) naive T cells to immune reconstitution and viral persistence is unknown. Methods. In a cross-sectional (n=94) and longitudinal (n=10) study of human immunodeficiency virus (HIV)-infected patients before and after ART, we examined the ratio of CD31(+) to CD31(-) naive CD4(+) T cells. In the longitudinal cohort we then quantified the concentration of HIV-1 DNA in each cell subset and performed single-genome amplification of virus from memory and naive T cells. Results. Patients receiving ART had a higher proportion of CD31(+) CD4(+) T cells than HIV-1-infected individuals naive to ART and uninfected control subjects (P<.001 and .007, respectively). After 24 months of ART, the proportion of CD31(+) naive CD4(+) T cells did not change, the concentration of HIV-1 DNA in memory CD4(+) T cells significantly decreased over time (P<.001), and there was no change in the concentration of HIV-1 DNA in CD31(+) or CD31(-) naive CD4(+) T cells (P=.751 and .251, respectively). Single-genome amplification showed no evidence of virus compartmentalization in memory and naive T cell subsets before or after ART. Conclusions. After ART, both CD31(+) and CD31(-) naive CD4(+) T cells expand, and both subsets represent a stable, persistent reservoir of HIV-1.
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