期刊
JOURNAL OF INFECTIOUS DISEASES
卷 200, 期 4, 页码 638-646出版社
OXFORD UNIV PRESS INC
DOI: 10.1086/600109
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资金
- Instituto Colombiano para el Desarrollo de la Ciencia y la Tecnologia Francisco Jose de Caldas [092-2006, 098-2007, 365-2006]
- United States National Institutes of Health (NIH)
- National Institute of Allergy and Infectious Diseases-ICDR [U19 AI065866]
- Fogarty International Center (NIH) [D43 TW006589]
Background. Leishmania (Viannia) species are the principal cause of mucosal leishmaniasis. The natural history and pathogenesis of mucosal disease are enigmatic. Parasitological evaluation of mucosal tissues has been constrained by the invasiveness of conventional sampling methods. Methods. We evaluated the presence of Leishmania in the mucosa of 26 patients with cutaneous leishmaniasis and 2 patients with mucocutaneous leishmaniasis. Swab samples of the nasal mucosa, tonsils, and conjunctiva were analyzed using polymerase chain reaction with LV-B1 primers and Southern blot hybridization. Results. Two patients with mucocutaneous leishmaniasis and 21 (81%) of 26 patients with cutaneous leishmaniasis had Leishmania kinetoplast minicircle DNA (kDNA) in mucosal tissues. kDNA was amplified from swab samples of nasal mucosa from 14 (58%) of 24 patients, tonsils from 13 (46%) of 28 patients, and conjunctiva from 6 (25%) of 24 patients. kDNA was detected in the mucosa of patients with cutaneous disease caused by Leishmania panamensis, Leishmania guyanensis, and Leishmania braziliensis. Conclusion. The asymptomatic presence of parasites in mucosal tissues may be common in patients with Leishmania (Viannia) infection.
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