期刊
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
卷 41, 期 9, 页码 1425-1433出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-014-1483-7
关键词
Klebsiella pneumonia; 2,3-Butanediol dehydrogenase/acetoin reductase; L-2,3-butanediol; Stereospecificity
资金
- Korea Research Foundation (KRF) for Basic Research Promotion - Korea Government (MEST)
- Joint Degree and Research Center - Korea Research Council of Fundamental Science Technology
Klebsiella pneumoniae synthesize large amounts of l-2,3-butanediol (l-2,3-BD), but the underlying mechanism has been unknown. In this study, we provide the first identification and characterization of an l-2,3-BD dehydrogenase from K. pneumoniae, demonstrating its reductive activities toward diacetyl and acetoin, and oxidative activity toward l-2,3-BD. Optimum pH, temperature, and kinetics determined for reductive and oxidative reactions support the preferential production of 2,3-BD during cell growth. Synthesis of l-2,3-BD was remarkably enhanced by increasing gene dosage, reaching levels that, to the best of our knowledge, are the highest achieved to date.
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