期刊
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
卷 40, 期 2, 页码 227-233出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-012-1224-8
关键词
Klebsiella pneumoniae; Glycerol; Ethanol; Aldehyde/alcohol dehydrogenase (AdhE)
资金
- Korea Ministry of Education, Science and Technology (Indo-Korea Joint Programme of Cooperation in Science Technology)
- Ministry of Knowledge Economy (International Joint Technology Commercialization Project)
- National Research Council of Science & Technology (NST), Republic of Korea [523300-13] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2011-0027171] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Transcriptome analysis of a K. pneumoniae GEM167 mutant strain derived by irradiation with gamma rays, which exhibited high-level production of ethanol from glycerol, showed that the mutant expressed AdhE at a high level. Ethanol production decreased significantly, from 8.8 to 0.5 g l(-1), when an adhE-deficient derivative of that strain was grown on glycerol. Bacterial growth was also reduced under such conditions, showing that AdhE plays a critical role in maintenance of redox balance by catalyzing ethanol production. Overexpression of AdhE enhanced ethanol production, from pure or crude glycerol, to a maximal level of 31.9 g l(-1) under fed-batch fermentation conditions; this is the highest level of ethanol production from glycerol reported to date.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据