期刊
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
卷 39, 期 10, 页码 1445-1452出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-012-1150-9
关键词
Cellulose degradation; Cellobiose; beta-glucosidase; Bio-ethanol; S. cerevisiae
资金
- South African National Energy Research Institute (SANERI)
- Department of Science and Technology (DST), South Africa
Recombinant Saccharomyces cerevisiae strains expressing beta-glucosidases from Thermoascus aurantiacus (Tabgl1) and Phanerochaete chrysosporium (PcbglB and Pccbgl1) were constructed and compared to S. cerevisiae Y294[SFI], previously identified as the best beta-glucosidase-producing strain. The PcbglB was also intracellularly expressed in combination with the lac12 lactose permease of Kluyveromyces lactis in S. cerevisiae Y294[PcbglB + Lac12]. The recombinant extracellular beta-glucosidases indicated maximum activity in the pH range 4-5 and temperature optima varying from 50 to 75 A degrees C. The S. cerevisiae Y294[Pccbgl1] strain performed best under aerobic and anaerobic conditions, producing 2.6 times more beta-glucosidase activity than S. cerevisiae Y294[SFI] and an ethanol concentration of 4.8 g l(-1) after 24 h of cultivation on cellobiose as sole carbohydrate source. S. cerevisiae Y294[Tabgl1] was unable to grow on cellobiose (liquid medium), whereas S. cerevisiae Y294[PcbglB + Lac12] exhibited limited growth.
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