期刊
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
卷 39, 期 11, 页码 1605-1613出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-012-1164-3
关键词
Atrazine; Bioremediation; Cell encapsulation; Electrospinning; Microtubes
资金
- Russell Berrie Nanotechnology Institute
- Stephen and Nancy Grand Water Research Institute
- BMBF-MOST German-Israeli Water Technology Research Fund [GR2336/103WT 1]
Electrospun hollow polymeric microfibers (microtubes) were evaluated as an encapsulation method for the atrazine degrading bacterium Pseudomonas sp. ADP. Pseudomonas sp. ADP cells were successfully incorporated in a formulation containing a core solution of polyethylene oxide dissolved in water and spun with an outer shell solution made of polycaprolactone and polyethylene glycol dissolved in a chloroform and dimethylformamide. The resulting microtubes, collected as mats, were partially collapsed with a ribbon-like structure. Following encapsulation, the atrazine degradation rate was low (0.03 +/- A 0.01 mg atrazine/h/g fiber) indicating that the electrospinning process negatively affected cell activity. Atrazine degradation was restored to 0.5 +/- A 0.1 mg atrazine/h/g fiber by subjecting the microtubes to a period of growth. After 3 and 7 days growth periods, encapsulated cells were able to remove 20.6 +/- A 3 and 47.6 +/- A 5.9 mg atrazine/g mat, respectively, in successive batches under non-growth conditions (with no additional electron donor) until atrazine was detected in the medium. The loss of atrazine degrading capacity was regained following an additional cell-growth period.
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