期刊
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
卷 38, 期 8, 页码 935-943出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-010-0864-9
关键词
Saccharomyces cerevisiae; Glycerol-3-phosphate dehydrogenase; NADP(+)-dependent glyceraldehyde-3-phosphate dehydrogenase; Glycerol production; Ethanol yield
资金
- Natural Science Foundation of China [20706024]
- 863'' Program [2006AA020101, 2007AA10Z359]
- Innovative Research Team of Jiangsu Province
- China Postdoctoral Science Foundation [200801361]
The GPD2 gene, encoding NAD(+)-dependent glycerol-3-phosphate dehydrogenase in an industrial ethanol-producing strain of Saccharomyces cerevisiae, was deleted. And then, either the non-phosphorylating NADP(+)-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN) from Bacillus cereus, or the NADP(+)-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Kluyveromyces lactis, was expressed in the obtained mutant AG2 deletion of GPD2, respectively. The resultant recombinant strain AG2A (gpd2 Delta P (PGK) -gapN) exhibited a 48.70 +/- A 0.34% (relative to the amount of substrate consumed) decrease in glycerol production and a 7.60 +/- A 0.12% (relative to the amount of substrate consumed) increase in ethanol yield, while recombinant AG2B (gpd2 Delta P (PGK) -GAPDH) exhibited a 52.90 +/- A 0.45% (relative to the amount of substrate consumed) decrease in glycerol production and a 7.34 +/- A 0.15% (relative to the amount of substrate consumed) increase in ethanol yield compared with the wild-type strain. More importantly, the maximum specific growth rates (mu (max)) of the recombinant AG2A and AG2B were higher than that of the mutant gpd2 Delta and were indistinguishable compared with the wild-type strain in anaerobic batch fermentations. The results indicated that the redox imbalance of the mutant could be partially solved by expressing the heterologous genes.
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