期刊
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
卷 38, 期 2, 页码 307-317出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-010-0774-x
关键词
Freshwater microalga Chlorella; Chlorophyll-fluorescence quenching; Photochemical activity; Biomass productivity; Thin-layer cascade unit; Hydrodynamic model
资金
- Italian CNR
- Czech Academy of Sciences
- Czech Science Foundation [522/06/1090, 521/09/0656]
- Ministry of Education [MSM6007665808]
- Czech Academy of Sciences [AVOZ 50200510]
- Italian MIUR (Ministero dell'Istruzione, Universita e Ricerca)
- FISR [prot. N. 1756 828.07.2005]
- Italian Space Agency [I/014/06/0]
This work aims to: (1) correlate photochemical activity and productivity, (2) characterize the flow pattern of culture layers and (3) determine a range of biomass densities for high productivity of the freshwater microalga Chlorella spp., grown outdoors in thin-layer cascade units. Biomass density, irradiance inside culture, pigment content and productivity were measured in the microalgae cultures. Chlorophyll-fluorescence quenching was monitored in situ (using saturation-pulse method) to estimate photochemical activities. Photobiochemical activities and growth parameters were studied in cultures of biomass density between 1 and 47 g L(-1). Fluorescence measurements showed that diluted cultures (1-2 g DW L(-1)) experienced significant photostress due to inhibition of electron transport in the PSII complex. The highest photochemical activities were achieved in cultures of 6.5-12.5 g DW L(-1), which gave a maximum daylight productivity of up to 55 g dry biomass m(-2) day(-1). A midday depression of maximum PSII photochemical yield (F (v)/F (m)) of 20-30% compared with morning values in these cultures proved to be compatible with well-performing cultures. Lower or higher depression of F (v)/F (m) indicated low-light acclimated or photoinhibited cultures, respectively. A hydrodynamic model of the culture demonstrated highly turbulent flow allowing rapid light/dark cycles (with frequency of 0.5 s(-1)) which possibly match the turnover of the photosynthetic apparatus. These results are important from a biotechnological point of view for optimisation of growth of outdoor microalgae mass cultures under various climatic conditions.
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