期刊
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
卷 37, 期 6, 页码 575-580出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-010-0703-z
关键词
Biosynthesis; Expression; Gene cluster; Pyrroloquinoline quinone
资金
- National Natural Science Foundation of China [20976053]
- National Basic Research Program (973) of China [2009CB724703]
We have expressed the pqqABCDE gene cluster from Gluconobacter oxydans, which is involved in pyrroloquinoline quinone (PQQ) biosynthesis, in Escherichia coli, resulting in PQQ accumulation in the medium. Since the gene cluster does not include the tldD gene needed for PQQ production, this result suggests that the E. coli tldD gene, which shows high homology to the G. oxydans tldD gene, carries out that function. The synthesis of PQQ activated d-glucose dehydrogenase in E. coli and the growth of the recombinant was improved. In an attempt to increase the production of PQQ, which acts as a vitamin or growth factor, we transformed E. coli with various recombinant plasmids, resulting in the overproduction of the PQQ synthesis enzymes and, consequently, PQQ accumulation-up to 6 mM-in the medium. This yield is 21.5-fold higher than that obtained in previous studies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据