期刊
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
卷 20, 期 4, 页码 1505-1510出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jiec.2013.07.039
关键词
AgNPs; B. marisflavi; UV-visible absorption Spectrum; ROS generation; Transmission electron microscopy
资金
- SMART-Research Professor Program of Konkuk University
- Konkuk University SMART-Full time Professorship
- GS Centre for Life Sciences
Silver nanoparticles (AgNPs) were prepared using culture supernatant of Bacillus marisflavi, a novel strain isolated from agricultural wastes. The formation of the AgNPs was confirmed using various analytical techniques such as UV-vis absorption spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy (TEM). The antibacterial effect of synthesized nanoparticles was investigated against Pseudomonas aeruginosa. Furthermore, the mechanism of action of cell death of P. aeruginosa was confirmed by reactive oxygen species (ROS) generation in AgNPs treated cells and also our results clearly indicate that AgNPs could induce generation of ROS by dose dependent manner. (C) 2013 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
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