期刊
JOURNAL OF IMMUNOLOGY
卷 192, 期 8, 页码 3719-3729出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1302960
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资金
- Top Institute Pharma [T4-102]
- FP7-founded European Virtual Institute of Malaria Research [242095]
- European Molecular Biology Organization
- European Vaccine Initiative
- European Malaria Vaccine Development Association Ph.D. scholarship
- Small Business Innovation Research Grants [R44AI058375-03, R44AI058375-04, R44AI058375-05]
- National Institute of Allergy and Infectious Diseases at the National Institutes of Health [05S1]
- Program for Appropriate Technology in Health (PATH) Malaria Vaccine Initiative [07984]
- Bill and Melinda Gates Foundation
Characteristic features of Plasmodium falciparum malaria are polyclonal B cell activation and an altered composition of the blood B cell compartment, including expansion of CD21(-)CD27(-) atypical memory B cells. BAFF is a key cytokine in B cell homeostasis, but its potential contribution to the modulation of the blood B cell pool during malaria remains elusive. In the controlled human malaria model (CHMI) in malaria-naive Dutch volunteers, we therefore examined the dynamics of BAFF induction and B cell subset activation and composition, to investigate whether these changes are linked to malaria-induced immune activation and, in particular, induction of BAFF. Alterations in B cell composition after CHMI closely resembled those observed in endemic areas. We further found distinct kinetics of proliferation for individual B cell subsets across all developmental stages. Proliferation peaked either immediately after blood-stage infection or at convalescence, and for most subsets was directly associated with the peak parasitemia. Concomitantly, plasma BAFF levels during CHMI were increased and correlated with membrane-expressed BAFF on monocytes and dendritic cells, as well as blood-stage parasitemia and parasite-induced IFN-gamma. Correlating with increased plasma BAFF and IFN-gamma levels, IgD(-)CD38(low)CD21(-)CD27(-) atypical B cells showed the strongest proliferative response of all memory B cell subsets. This provides unique evidence for a link between malaria-induced immune activation and temporary expansion of this B cell subset. Finally, baseline BAFF-R levels before CHMI were predictive of subsequent changes in proportions of individual B cell subsets. These findings suggest an important role of BAFF in facilitating B cell subset proliferation and redistribution as a consequence of malaria-induced immune activation.
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