期刊
JOURNAL OF IMMUNOLOGY
卷 190, 期 12, 页码 6389-6396出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1300365
关键词
-
类别
资金
- Biotechnology and Biological Sciences Research Council [B/G004013/1]
- Biotechnology and Biological Sciences Research Council [BB/G004013/1, BBS/E/D/20251969, BBS/E/D/20310000] Funding Source: researchfish
- Medical Research Council [MR/K001744/1] Funding Source: researchfish
- BBSRC [BBS/E/D/20310000, BB/G004013/1, BBS/E/D/20251969] Funding Source: UKRI
- MRC [MR/K001744/1] Funding Source: UKRI
Human and mouse monocyte can be divided into two different subpopulations based on surface marker expression: CD14/16 and Ly6C/CX3CR1, respectively. Monocyte subpopulations in the pig were identified based on reciprocal expression of CD14 and the scavenger receptor CD163. The two populations, CD14(hi) - CD163(low) and CD14(low) - CD163(hi), show approximately equal abundance in the steady- state. Culture of pig PBMCs in CSF1 indicates that the two populations are a maturation series controlled by this growth factor. Gene expression in pig monocyte subpopulations was profiled using the newly developed and annotated pig whole genome snowball microarray. Previous studies have suggested a functional equivalence between human and mouse subsets, but certain genes such as CD36, CLEC4E, or TREM-1 showed human- specific expression. The same genes were expressed selectively in pig monocyte subsets. However, the profiles suggest that the pig CD14(low) - CD163(high) cells are actually equivalent to intermediate human monocytes, and there is no CD14(-) CD16(+) nonclassical'' population. The results are discussed in terms of the relevance of the pig as a model for understanding human monocyte function.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据