期刊
JOURNAL OF IMMUNOLOGY
卷 191, 期 3, 页码 1188-1199出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1300739
关键词
-
类别
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [20689005, 22790194, 22370072, 23113517, 24111005, 22111003]
- Core Research for Evolutionary Science and Technology (CREST) grant from the Japan Science and Technology Agency
- Takeda Science Foundation
- Grants-in-Aid for Scientific Research [25670101] Funding Source: KAKEN
T lymphocytes vigorously migrate within the paracortex of lymph nodes (LNs) in search of cognate Ags that are presented by dendritic cells (DCs). However, the mechanisms that support T cells to exert the highest motility in a densely packed LN microenvironment are not fully understood. Two-photon microscopy using LN tissue slices revealed that LFA-1 and ICAM-1 were required for high-velocity migration (>10 mu m/min) with relatively straight movement. Importantly, ICAM-1 expressed by myeloid lineages, most likely DCs, but not stromal cells or lymphocytes, was sufficient to support the high-velocity migration. Visualizing DCs in the LN from CD11c-EYFP mice showed that T cells traveled over thin dendrites and the body of DCs. Interestingly, DCs supported T cell motility in vitro in chemokine- and ICAM-1-dependent manners. Moreover, an acute lymphopenic environment in the LN significantly increased LFA-1 dependency for T cell migration, indicating that lymphocyte density modulates the use of LFA-1. Therefore, our results indicate that LFA-1/ICAM-1-dependent interactions between T cells and DCs play a crucial role not only in supporting firm arrest during Ag recognition but also in facilitating the Ag scanning processes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据