期刊
JOURNAL OF IMMUNOLOGY
卷 189, 期 7, 页码 3557-3565出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1200212
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资金
- National Science Fund for Distinguished Young Scholars [30725029]
- National Basic Research Program of China (973 Program) [2010CB945300]
- Taishan Scholar Foundation of Shandong Province
Mammalian IgG and IgE are thought to have evolved from IgY of nonmammalian tetrapods; however, no diversification of IgY subclasses has been reported in reptiles or birds, which are phylogenetically close to mammals. To our knowledge, we report the first evidence of the presence of multiple IgY-encoding (v) genes in snakes. Two v genes were identified in the snake Elaphe taeniura, and three v genes were identified in the Burmese python (Python molurus bivittatus). Although four of the v genes displayed a conventional four-H chain C region exon structure, one of the v genes in the Burmese python lacked the H chain C region 2 exon, thus exhibiting a structure similar to that of the mammalian gamma genes. We developed mouse mAbs specific for the IgY1 and IgY2 of E. taeniura and showed that both were expressed in serum; each had two isoforms: one full-length and one truncated at the C terminus. The truncation was not caused by alternative splicing or transcriptional termination. We also identified the mu and delta genes, but no a gene, in both snakes. This study provides valuable clues for our understanding of Ig gene evolution in tetrapods. The Journal of Immunology, 2012, 189: 3557-3565.
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