期刊
JOURNAL OF IMMUNOLOGY
卷 189, 期 6, 页码 3085-3091出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1200821
关键词
-
类别
资金
- National Institutes of Health [R01 DE018503, R01 DK091191]
Clostridium difficile is a Gram-positive obligate anaerobic pathogen that causes pseudomembranous colitis in antibiotic-treated individuals. Commensal bacteria are known to have a significant role in the intestinal accumulation of C. difficile after antibiotic treatment, but little is known about how they affect host immunity during C. difficile infection. In this article, we report that C. difficile infection results in translocation of commensals across the intestinal epithelial barrier that is critical for neutrophil recruitment through the induction of an IL-1 beta-mediated positive-feedback loop. Mice lacking ASC, an essential mediator of IL-1 beta and IL-18 processing and secretion, were highly susceptible to C. difficile infection. ASC(-/-) mice exhibited enhanced translocation of commensals to multiple organs after C. difficile infection. Notably, ASC(-/-) mice exhibited impaired CXCL1 production and neutrophil influx into intestinal tissues in response to C. difficile infection. The impairment in neutrophil recruitment resulted in reduced production of IL-1 beta and CXCL1 but not IL-18. Importantly, translocated commensals were required for ASC/Nlrp3-dependent IL-1 beta secretion by neutrophils. Mice lacking IL-1 beta were deficient in inducing CXCL1 secretion, suggesting that IL-1 beta is the dominant inducer of ASC-mediated CXCL1 production during C. difficile infection. These results indicate that translocated commensals play a crucial role in CXCL1-dependent recruitment of neutrophils to the intestine through an IL-1 beta/NLRP3/ASC-mediated positive-feedback mechanism that is important for host survival and clearance of translocated commensals during C. difficile infection. The Journal of Immunology, 2012, 189:3085-3091.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据