期刊
JOURNAL OF IMMUNOLOGY
卷 187, 期 6, 页码 3362-3373出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1101235
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资金
- National Heart, Lung, and Blood Institute [F30HL104892]
- Albert J. Ryan Foundation
- National Institutes of Health [T32HD046387, R01AI083450]
- National Institute of Diabetes and Digestive and Kidney Diseases [P30DK049216]
- Campaign Urging Research for Eosinophilic Disease
- Buckeye Foundation
- Food Allergy Project/Food Allergy Initiative
An altered balance between Th1 and Th2 cytokines is responsible for a variety of immunoinflammatory disorders such as asthma, yet the role of posttranscriptional mechanisms, such as those mediated by microRNAs (miRs), in adjusting the relative magnitude and balance of Th cytokine expression have been largely unexplored. In this study, we show that miR-21 has a central role in setting a balance between Th1 and Th2 responses to Ags. Targeted ablation of miR-21 in mice led to reduced lung eosinophilia after allergen challenge, with a broadly reprogrammed immunoactivation transcriptome and significantly increased levels of the Th1 cytokine IFN-gamma. Biological network-based transcriptome analysis of OVA-challenged miR-21(-/-) mice identified an unexpected prominent dysregulation of IL-12/IFN-gamma pathways as the most significantly affected in the lungs, with a key role for miR-21 in IFN-g signaling and T cell polarization, consistent with a functional miR-21 binding site in IL-12p35. In support of these hypotheses, miR-21 deficiency led dendritic cells to produce more IL-12 after LPS stimulation and OVA-challenged CD4(+) T lymphocytes to produce increased IFN-gamma and decreased IL-4. Further, loss of miR-21 significantly enhanced the Th1-associated delayed-type hypersensitivity cutaneous responses. Thus, our results define miR-21 as a major regulator of Th1 versus Th2 responses, defining a new mechanism for regulating polarized immunoinflammatory responses. The Journal of Immunology, 2011, 187: 3362-3373.
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