期刊
JOURNAL OF IMMUNOLOGY
卷 184, 期 8, 页码 4414-4422出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.0903332
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资金
- Ministry of Education, Culture Sports, Science and Technology of Japan
- Japan Society for Promotion of Science
- Naito Foundation
- Takeda Science Foundation
Granulomas play an essential role in the sequestration and killing of mycobacteria in the lung; however, the mechanisms of their development and maturation are still not clearly understood. IL-17A is involved in mature granuloma formation in the mycobacteria-infected lung. Therefore, IL-17A gene-knockout (KO) mice fail to develop mature granulomas in the Mycobacterium bovis bacille Calmette-Guerin (BCG)-infected lung. This study analyzed the mechanism of IL-17A-dependent mature granuloma formation in the mycobacteria-infected lung. The IL-17A KO mice showed a normal level of nascent granuloma formation on day 14 but failed to develop mature granulomas on day 28 after the BCG infection in the lung. The observation implies that IL-17A is required for the maturation of granuloma from the nascent to mature stage. TCR gamma delta T cells expressing TCR V gamma 4 or V gamma 6 were identified as the major IL-17A-producing cells that resided in the BCG-induced lung granuloma. The adoptive transfer of the IL-17A-producing TCR gamma delta T cells reconstituted granuloma formation in the IL-17A KO mice. The expression of ICAM-1 and LFA-1, which are adhesion molecules important in granuloma formation, decreased in the lung of the BCG-infected IL-17A KO mice, and their expression was induced on BCG-infected macrophages in coculture with IL-17A-producing TCR gamma delta T cells. Furthermore, IL-17A KO mice showed not only an impaired mature granuloma formation, but also an impaired protective response to virulent Mycobacterium tuberculosis. Therefore, IL-17A produced by TCR gamma delta T cells plays a critical role in the prevention of M. tuberculosis infection through the induction of mature granuloma formation. The Journal of Immunology, 2010, 184: 4414-4422.
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