期刊
JOURNAL OF IMMUNOLOGY
卷 185, 期 7, 页码 4042-4052出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1001730
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资金
- Biotechnology and Biological Sciences Research Council
- Medical Research Council
- Biotechnology and Biological Sciences Research Council [BBS/B/02010, BB/E021638/1] Funding Source: researchfish
- Medical Research Council [G0601618] Funding Source: researchfish
- BBSRC [BB/E021638/1] Funding Source: UKRI
- MRC [G0601618] Funding Source: UKRI
The generation of high-affinity Abs is essential for immunity and requires collaboration between B and T cells within germinal centers (GCs). By using novel mouse models with a conditional deletion of the p110 delta catalytic subunit of the PI3K pathway, we established that p110d is required in T cells, but not in B cells, for the GC reaction. We found the formation of T follicular helper (T(FH)) cells to be critically dependent on p110d in T cells. Furthermore, by deleting phosphatase and tensin homolog deleted on chromosome 10, which opposes p110d in activated T cells, we found a positive correlation between increased numbers of T(FH) cells and GC B cells. These results are consistent with the hypothesis that T cell help is the limiting factor in the GC reaction. P110 delta was not required for the expression of B cell lymphoma 6, the downregulation of CCR7, or T cell entry into primary follicles. Instead, p110d was the critical catalytic subunit for ICOS downstream signaling and the production of key T(FH) cytokines and effector molecules. Our findings support a model in which the magnitude of the GC reaction is controlled by the activity of the PI3K pathway in T(FH) cells. The Journal of Immunology, 2010, 185: 4042-4052.
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