期刊
JOURNAL OF IMMUNOLOGY
卷 185, 期 7, 页码 4292-4301出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1000650
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资金
- South African National Research Foundation
- Medical Research Council
- National Health and Laboratory Services
- Centre National de la Recherche Scientifique
- Fondation de la Recherche Medicale
- European Union [028190]
- Howard Hughes Medical Institute
- Swiss National Foundation for Scientific Research [3200A0-118196]
The contribution of lymphotoxin (LT)alpha in the host immune response to virulent Mycobacterium tuberculosis and Mycobacterium bovis bacillus Calmette-Guerin infections was investigated. Despite their ability to induce Th1 cytokine, IFN-gamma, and IL-12 pulmonary response, conventional LTa-/- mice succumb rapidly to virulent M. tuberculosis aerosol infection, with uncontrolled bacilli growth, defective granuloma formation, necrosis, and reduced pulmonary inducible NO synthase expression, similar to TNF-/- mice. Contributions from developmental lymphoid abnormalities in LTa-/- mice were excluded because hematopoietic reconstitution with conventional LTa-/- bone marrow conferred enhanced susceptibility to wild-type mice, comparable to conventional LTa-/- control mice. However, conventional LTa-/- mice produced reduced levels of TNF after M. bovis bacillus Calmette-Guerin infection, and their lack of control of mycobacterial infection could be due to a defective contribution of either LT alpha or TNF, or both, to the host immune response. To address this point, the response of neo-free LTa-/- mice with unperturbed intrinsic TNF expression to M. tuberculosis infection was investigated in a direct comparative study with conventional LTa-/- mice. Strikingly, although conventional LTa-/- mice were highly sensitive, similar to TNF-/- mice, neo-free LTa-/- mice controlled acute M. tuberculosis infection essentially as wild-type mice. Pulmonary bacterial burden and inflammation was, however, slightly increased in neo-free LTa-/- mice 4-5 mo postinfection, but importantly, they did not succumb to infection. Our findings revise the notion that LTa might have a critical role in host defense to acute mycobacterial infection, independent of TNF, but suggest a contribution of LTa in the control of chronic M. tuberculosis infection. The Journal of Immunology, 2010, 185: 4292-4301.
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