期刊
JOURNAL OF IMMUNOLOGY
卷 185, 期 7, 页码 4272-4283出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1000874
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资金
- National Institutes of Health [R01 EY14362, P30 EY11373, DE14924]
- American Cancer Society [RSG-09-198-01-MPC]
- Research to Prevent Blindness Foundation
- Ohio Lions Eye Research Foundation
Pseudomonas aeruginosa is a major cause of blindness and visual impairment in the United States and worldwide. Using a murine model of keratitis in which abraded corneas are infected with P. aeruginosa parent and Delta fliC (aflagellar) strains 19660 and PAO1, we found that F4/80(+) macrophages were the predominant cell type in the cornea expressing TLR2, TLR4, and TLR5. Depletion of macrophages and dendritic cells using transgenic Mafia mice, in which Fas ligand is selectively activated in these cells, resulted in diminished cytokine production and cellular infiltration to the corneal stroma and unimpaired bacterial growth. TLR4(-/-) mice showed a similar phenotype postinfection with Delta fliC strains, whereas TLR4/5(-/-) mice were susceptible to corneal infection with parent strains. Bone marrow-derived macrophages stimulated with Delta fliC bacteria induced Toll/IL-1R intracellular domain (TIR)containing adaptor inducing IFN-beta (TRIF)-dependent phosphorylation of IFN regulatory factor 3 in addition to TIR-containing adaptor protein/MyD88-dependent phosphorylation of I kappa B and nuclear translocation of the p65 subunit of NF kappa B. Furthermore, TRIF(-/-) mice showed a similar phenotype as TLR4(-/-) mice in regulating only Delta fliC bacteria, whereas MyD88(-/-) mice were unable to clear parent or Delta fliC bacteria. Finally, IL-1R1(-/-) and IL-1 alpha/beta(-/-) mice were highly susceptible to infection. Taken together, these findings indicate that P. aeruginosa activates TLR4/5 on resident corneal macrophages, which signal through TRIF and TIR-containing adaptor protein/MyD88 pathways, leading to NF-kappa B translocation to the nucleus, transcription of CXCL1 and other CXC chemokines, recruitment of neutrophils to the corneal stroma, and subsequent bacterial killing and tissue damage. IL-1 alpha and IL-1 beta are also produced, which activate an IL-1R1/MyD88-positive feedback loop in macrophages and IL-1R on other resident cells in the cornea. The Journal of Immunology, 2010, 185: 4272-4283.
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