期刊
JOURNAL OF IMMUNOLOGY
卷 185, 期 12, 页码 7367-7373出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1001798
关键词
-
类别
资金
- Bundesministerium fur Bildung und Forschung Biofuture, GoBio [SFB704, SFB670, SFB832, KFO177]
- Deutsche Forschungsgemeinschaft [BA 3544/1-1]
Bacterial DNA contains unmethylated CpG dinucleotides and is a potent ligand for TLR9. Bacterial DNA has been claimed the active ingredient in bacterial lysates used for immunotherapy. Whereas the detection of viral DNA by TLR9 expressed in plasmacytoid dendritic cells (PDCs) with subsequent IFN-alpha production is well defined, the role of bacterial DNA during microbial infection is less clear. In fact, IFN-alpha is not a hallmark of antibacterial immune responses. Unlike in mice, TLR9 expression in humans is restricted to PDCs and B cells; thus, conclusions from murine models of infection have limitations. In this study, we demonstrate that lysates of heat-killed Escherichia coli containing bacterial DNA induced IFN-alpha in isolated PDCs but not in the mixed cell populations of human PBMCs. Depletion of monocytes restored IFN-alpha secretion by PDCs within PBMCs. We found that monocyte-derived IL-10 and PGs contribute to monocyte-mediated inhibition of IFN-alpha release in PDCs. We conclude that human PDCs can be stimulated by bacterial DNA via TLR9; however, in the physiological context of mixed-cell populations, PDC activation is blocked by factors released from monocytes stimulated in parallel by other components of bacterial lysates such as LPS. This functional repression of PDCs by concomitantly stimulated monocytes avoids production of antiviral IFN-alpha during bacterial infection and thus explains how the innate immune system is enabled to distinguish bacterial from viral CpG DNA and thus to elicit the appropriate responses despite the presence of CpG DNA in both types of infection. The Journal of Immunology, 2010, 185: 7367-7373.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据