期刊
JOURNAL OF IMMUNOLOGY
卷 184, 期 12, 页码 6920-6928出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.0904024
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资金
- French Association pour la Recherche sur le Cancer
- Ligue Nationale contre le Cancer [R07002BBA]
- Institut National de la Sante et de la Recherche Medicale (Avenir)
- Fondation pour la Recherche Medicale, respectively
Human V gamma 9V delta 2 T lymphocytes are activated by phosphoantigens provided exogenously or produced by tumors and infected cells. Activation requires a contact between V gamma 9V delta 2 cells and neighboring cells. We previously reported a role for cell surface F-1-adenosine triphosphatase (ATPase) in T cell activation by tumors and specific interactions between V gamma 9V delta 2 TCRs and purified F-1-ATPase. 721.221 cells do not express surface F-1-ATPase and do not support phosphoantigen responses unless they are rendered apoptotic by high doses of zoledronate, a treatment that promotes F-1-expression as well as endogenous phosphoantigen production. By monitoring calcium flux in single cells, we show in this study that contact of T cells with F-1-ATPase on polystyrene beads can partially replace the cell-cell contact stimulus during phosphoantigen responses. Triphosphoric acid 1-adenosin-5'-yl ester 3-(3-methylbut-3-enyl) ester, an adenylated derivative of isopentenyl pyrophosphate, can stably bind to F-1-ATPase-coated beads and promotes TCR aggregation, lymphokine secretion, and activation of the cytolytic process provided that nucleotide pyrophosphatase activity is present. It also acts as an allosteric activator of F-1-ATPase. In the absence of V gamma 9V delta 2 cells, triphosphoric acid 1-adenosin-5'-yl ester 3-(3-methylbut-3-enyl) ester immobilized on F-1-ATPase is protected from nucleotide pyrophosphatase activity, as is the antigenic activity of stimulatory target cells. Our experiments support the notion that V gamma 9V delta 2 T cells are dedicated to the recognition of phosphoantigens on cell membranes in the form of nucleotide derivatives that can bind to F-1-ATPase acting as a presentation molecule. The Journal of Immunology, 2010, 184: 6920-6928.
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