期刊
JOURNAL OF IMMUNOLOGY
卷 183, 期 7, 页码 4619-4627出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.0804024
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资金
- National Institutes of Health, National Institute of Allergy and Infectious Diseases [142334, D43 TW007127]
Control of infection caused by Leishmania major requires the development of IFN-gamma(+)CD4(+) lymphocytes for the induction of microbicidal activity in host macrophages. We recently reported on the inability of conventionally resistant C57BL/6 mice to successfully resolve infection by an isolate of L. major, despite a strong IFN-gamma response by the host. Susceptibility was caused by Ag-specific IL-10 from CD4(+) cells that were also producing IFN-gamma. In the present studies, we have explored the role for IL-27 in the regulation of IL-10 from Th1 cells in leishmaniasis. Cytokine analysis of CD4(+) cells in the lesions and draining lymph nodes of infected IL-27R-deficient (WSX-1(-/-)) mice revealed diminished IL-10 from IFN-gamma(+) CD4(+) cells, which was accompanied by a reduction in total IFN-gamma(+)CD4(+) cells and an increase in IL-4. Despite the inhibition of IL-10 from CD4(+) cells, no significant change in parasite numbers was observed, due both to the shift in the Th1/Th2 balance and to residual levels of IL-10. Strikingly, infected WSX-1(-/-) mice developed more severe lesions that were associated with the appearance of IL-17(+) CD4(+) cells, demonstrating a function for IL-27 in blocking the development of inappropriate Th17 cells during L. major infection. The results demonstrate the pleiotropic effects that IL-27 has on L. major-driven Th1, Th2, and Th17 development, and reinforce its function as a key regulatory cytokine that controls the balance between immunity and pathology. The Journal of Immunology, 2009, 183: 4619-4627.
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