期刊
JOURNAL OF IMMUNOLOGY
卷 183, 期 7, 页码 4800-4808出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.0900903
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资金
- Cancer Vaccine Collaborative
- Cancer Research Institute
- Ludwig Institute for Cancer Research.
Vaccination with cancer/testis Ag MAGE-A3 in the form of recombinant protein often induces specific humoral and cellular immune responses. Although Ag-specific CD4(+) T cells following vaccination are detectable by cytokine production after a single in vitro stimulation, their detection before vaccination is difficult because of low frequency. In this study, we have applied a sensitive method using CD154 (CD40L) staining to detect NIAGE-A3-specific CD4(+) T cells. MAGE-A3-specific T cell responses were analyzed in four healthy donors, two lung cancer patients with spontaneous serum Abs to MAGE-A3, and two baseline seronegative lung cancer patients throughout vaccination with MAGE-A3 protein. NIAGE-A3-specific CD4(+) T cells were detected in all individuals tested, at low frequency in healthy donors and seronegative cancer patients and higher frequency in patients seropositive for MAGE-A3. Polyclonal expansion of CD154-expressing CD4(+) T cells after cell sorting generated a large number of MAGE-A3-specific CD4(+) T cell lines from all individuals tested, enabling full characterization of peptide specificity, HLA-restriction, and avidity. Application of this method to cancer patients vaccinated with MAGE-A3 protein with or without adjuvant revealed that protein vaccination induced oligoclonal activation of MAGE-A3-specific CD4(+) T cells. It appeared that MAGE-A3 protein vaccination in the presence of adjuvant selectively expanded high avidity CD4(+) T cells, whereas high avidity T cells disappeared after multiple vaccinations with MAGE-A3 protein alone. The Journal of Immunology, 2009,183: 4800-4808.
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