期刊
JOURNAL OF IMMUNOLOGY
卷 181, 期 11, 页码 7936-7943出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.181.11.7936
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资金
- National Institutes of Health [HL-06931, HD28827, HL-44015, HL-29594]
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [P01HL029594, R01HL044015, K08HL089505] Funding Source: NIH RePORTER
Surfactant protein D (SP-D) plays important roles in the initial innate defense against influenza A virus (IAV). The collagen domain of SP-D is probably critical for its homeostatic functions in vivo and has been implicated in the modulation of macrophage responses to SP-D-ligand complexes. For the current studies, we used a panel of rat SP-D mutants lacking all or part of the collagen domain to more specifically evaluate the contributions of this domain to viral interactions. SP-D multimers lacking the collagenous sequence efficiently neutralized Phil82 IAV, promoted neutrophil uptake of IAV, and also potentiated the IAV-induced neutrophil respiratory burst response. A dodecameric mutant with shortened collagenous arms showed enhanced viral aggregation and neuraminidase inhibition, and an increased capacity to inhibit a partially collectin-resistant strain of IAV. By contrast, truncated molecules lacking an N-terminal and collagen domain showed no detectable antiviral and opsonizing activity, despite preservation of lectin activity and detectable viral binding. Thus, multimerization, which is mediated by the N-peptide, is more important than the collagen domain for efficient viral neutralization and opsonization. However, the structure of the collagen domain significantly influences the anti-viral activity of multimerized forms of SP-D. The Journal of Immunology, 2008, 181: 7936-7943.
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