期刊
JOURNAL OF IMMUNOLOGY
卷 181, 期 1, 页码 383-392出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.181.1.383
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- NCI NIH HHS [R01 CA098129-01A2, R01 CA098129-04, R01 CA098129-03, R01 CA098129-05, R01 CA098129-02, R01 CA098129, CA098129] Funding Source: Medline
- NIAID NIH HHS [R01 AI056123-03, R01 AI056123, AI52077, R01 AI056123-05, R01 AI056123-01A1, R01 AI056123-02, R01 AI056123-04, AI056123, T32 AI052077] Funding Source: Medline
IL-7 plays a critical role in B cell fate decision by regulating early B cell factor (EBF) expression. However, it was not clear when IL-7 stimulation is necessary in hemato-/lymphopoiesis in adult mice. Here we show that pre-proB cells derived from IL-7(-/-) mice have lost B cell potential, despite up-regulation of EBF expression following IL-7 stimulation. Pre-proB cells from wild-type mice can give rise to proB cells in the absence of IL-7. In this case, EBF up-regulation during the transition from the pre-proB to proB stages occurs normally. In contrast, EBF expression by IL-7(-/-) pre-proB cells after IL-7 stimulation is similar to 20 times lower than wild-type pre-proB cells. In addition, only multipotent progenitors with higher levels of ectopic EBF can give rise to proB cells in the absence of IL-7. Therefore, the primary function of IL-7 before the pre-proB stage in B cell development is to maintain the EBF expression level above a certain threshold, which is necessary for pre-proB cells to further transit to the proB stage.
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