期刊
JOURNAL OF IMMUNOLOGY
卷 181, 期 2, 页码 1170-1178出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.181.2.1170
关键词
-
类别
资金
- NHLBI NIH HHS [HL-61005, HL-36577] Funding Source: Medline
Dendritic cells (DC) are important APCs that control allergen-induced airway responses by interacting directly with T cells. Leukotriene B(4) (LTB(4)), interacting with its high-affinity receptor, LTB4 receptor 1 (BLT1), is known to attract and activate leukocytes during inflammation. We have previously shown that BLT1 expression on Ag-primed T cells is required for the development of airway hyperresponsiveness (AHR; Miyahara et al. 2005. Am. J. Respir. Crit. Care Med. 172: 161-167). However, the role for the LTB(4)-BLT1 pathway in DC function in allergen-induced airway responses has not been defined. Bone marrow-derived DCs (BMDC) were generated. Naive BALB/c mice received OVA-pulsed BLT1-deficient (BLTI(-/-)) BMDCs or wild-type BMDCs intratracheally and were then challenged with OVA for 3 days. Airway responses were monitored 48 h after the last allergen challenge. BLTI(-/-) BMDCs showed normal maturation judged from surface expression of CD markers. Compared with recipients of wild-type BMDCs, mice that received BLT1(-/-) BMDCs developed significantly lower AHR to inhaled methacholine, lower goblet cell metaplasia, and eosinophilic infiltration in the airways and decreased levels of Th2 type cytokines in the bronchoalveolar lavage fluid. Migration of BLTI(-/-) BMDCs into peribronchial lymph nodes was significantly impaired compared with BLTI(+/+) BMDCs after intratracheal instillation. These data suggest that BLTI expression on DCs is required for migration of DCs to regional lymph nodes as well as in the development of AHR and airway inflammation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据