期刊
JOURNAL OF IMMUNOLOGY
卷 180, 期 10, 页码 6611-6622出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.180.10.6611
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- NIAID NIH HHS [AI 42396] Funding Source: Medline
We examined the TCR repertoire used by naive SJL mice in their in vitro spontaneous response to proteolipid protein (PLP) 139-151 by V beta-J beta spectratyping and compared it to that used after immunization with the peptide. T cells from immunized mice use the public rearrangement V beta 10-J beta 1.1, but naive mice do not; in contrast, TCR CDR3-beta rearrangements of V beta 18-J beta 1.2 and V beta 19-J beta 1.2 consistently are associated with the spontaneous response. T cells involved in spontaneous and induced responses can each recognize PLP139-151 presented in vivo, but its s.c. administration has different consequences for the two repertoires. Four days after immunization, T cells associated with spontaneous responsiveness appear in the draining lymph nodes but disappear by day 10 and never appear elsewhere. Simultaneously, V beta 10-J beta 1.1 T cells are likewise activated in the lymph nodes by day 4 and spread to the spleen by day 10. Eight- to 10-wk-old naive mice use a narrower repertoire or TCRs than do immunized age-matched mice. Induced V beta 10-J beta 1.1 T cells home to the CNS during experimental autoimmune encephalomyelitis, whereas we failed to detect V beta 18-J beta 1.2 and V beta 19-J beta 1.2 TCR rearrangements in the CNS. Thus, we observe that administration of PLP139-151 primes a T cell repertoire distinct from the one responsible for spontaneous responsiveness. This immunized repertoire substitutes for the naive one and becomes dominant at the time of disease onset.
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