期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 387, 期 1-2, 页码 270-275出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2012.11.004
关键词
Contact hypersensitivity; DNFB; Ear-swelling; Vascular permeability; MPO; IFN-gamma
资金
- Ministry of Science and Higher Education [N N401 545940, N N401 006939, N N401 000936, 2011/01/B/NZ6/00300, K/ZDS/002182]
Contact hypersensitivity (CHS) is an experimental model of allergic contact dermatitis (ACD) that can be studied in mice. For CHS responses, mice are immunized by painting with a reactive hapten, such as 1-fluoro-4,6-dinitrobenzene (DNFB), on the shaved abdominal and chest skin. Subsequently, the ears are challenged with diluted hapten, eliciting 'hypersensitive' ear-swelling reactions, which can be measured with a micrometer. In this manuscript we present complementary methods that can be used to evaluate CHS in mice that include: ear weight vascular permeability, myeloperoxidase (MPO) activity, IFN-gamma concentration in ear extracts and also IFN-gamma production by auricular lymph node cells (ELNC). The biochemical evaluation of CHS can be also supported by proliferation assay, measurement of IFN-gamma production by skin-draining lymph node cells employing ELISA test and by evaluation of IFN-gamma(+) TCR alpha beta CD8 cells with the use of flow cytometry. (c) 2012 Elsevier B.V. All rights reserved.
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