期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 375, 期 1-2, 页码 182-188出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2011.10.010
关键词
Collectin 11; Collectin kidney 1; Complement; Innate immunity; Enzyme-linked immunosorbent assay; 3MC syndrome
资金
- A.P. Moeller Foundation
- The Danish Arthritis Association
- Danielsen's Foundation
- Foundation of 1870
- The Lundbeck Foundation
- The Danish Medical Research Council
- NEWLIFE
- MRC [G0802138] Funding Source: UKRI
- Medical Research Council [G0802138] Funding Source: researchfish
Collectin 11 (CL-11), also referred to as collectin kidney 1 (CL-K1), is a pattern recognition molecule that belongs to the collectin group of proteins involved in innate immunity. It interacts with glycoconjugates on pathogen surfaces and has been found in complex with mannose-binding lectin-associated serine protease 1 (MASP-1) and/or MASP-3 in circulation. Mutation in the CL-11 gene was recently associated with the developmental syndrome 3MC. In the present study, we established and thoroughly validated a sandwich enzyme-linked immunosorbent assay (ELISA) based on two different monoclonal antibodies. The assay is highly sensitive, specific and shows excellent quantitative characteristics such as reproducibility, dilution linearity and recovery (97.7-104%). The working range is 0.15-34 ng/ml. The CL-11 concentration in two CL-11-deficient individuals affected by the 3MC syndrome was determined to be below 2.1 ng/ml. We measured the mean serum CL-11 concentration to 284 ng/ml in 100 Danish blood donors, with a 95% confidence interval of 269-299 ng/ml. There was no significant difference in the CL-11 concentration measured in matched serum and plasma samples. Storage of samples and repeated freezing and thawing to a certain extent did not influence the ELISA. This ELISA offers a convenient and reliable method for studying CL-11 levels in relation to a variety of human diseases and syndromes. (C) 2011 Elsevier B.V. All rights reserved.
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