期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 374, 期 1-2, 页码 5-12出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2010.10.012
关键词
MHC-I; Peptide; Stability; Dissociation; Scintillation proximity assay
资金
- National Institute of Health [HHSN266200400025C, HHSN272200900045C]
Efficient presentation of peptide-MHC class I complexes to immune T cells depends upon stable peptide-MHC class I interactions. Theoretically, determining the rate of dissociation of a peptide-MHC class I complexes is straightforward; in practical terms, however, generating the accurate and closely timed data needed to determine the rate of dissociation is not simple. Ideally, one should use a homogenous assay involving an inexhaustible and label-free assay principle. Here, we present a homogenous, high-throughput peptide-MHC class I dissociation assay, which by and large fulfill these ideal requirements. To avoid labeling of the highly variable peptide, we labeled the invariant beta 2m and monitored its dissociation by a scintillation proximity assay, which has no separation steps and allows for real-time quantitative measurement of dissociation. Validating this work-around to create a virtually label-free assay, we showed that rates of peptide-MHC class I dissociation measured in this assay correlated well with rates of dissociation rates measured conventionally with labeled peptides. This assay can be used to measure the stability of any peptide-MHC class I combination, it is reproducible and it is well suited for high-throughput screening. To exemplify this, we screened a panel of 384 high-affinity peptides binding to the MHC class I molecule, HLA-A*02:01, and observed the rates of dissociation that ranged from 0.1 h to 46 h depending on the peptide used. (C) 2010 Elsevier B.V. All rights reserved.
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