期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 359, 期 1-2, 页码 1-10出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2010.04.004
关键词
Mouse; Monocyte; Purification; Peripheral blood; Immuno-magnetic labeling
资金
- Fund for Scientific Research Flanders (FWO-Vlaanderen)
- Institute for the Promotion of Innovation by Science and Technology in Flanders (IWT)
Studies in mice have advanced our understanding of the contribution of monocytes to inflammation and immunity. However, the lack of a straightforward and reliable method for the isolation of mouse blood monocytes remains a hurdle. Here we describe a fast and easy method for isolating monocytes from mouse blood based on immuno-magnetic labeling. By negative selection we were able to isolate enriched fractions (>50% purity) of unlabeled monocytes. A subsequent positive selection step resulted in purities of >= 90% viable and functional monocytes. This method is of general use for studying mouse monocyte biology. (C) 2010 Elsevier B.V. All rights reserved.
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