期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 348, 期 1-2, 页码 9-17出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2009.06.004
关键词
Prostate cancer; Androgen-deprivation therapy; Immunohistochemistry; Computer-assisted quantification; Tumor-infiltrating lymphocytes; NK cells; Macrophages
资金
- Canadian Uro-Oncology/AstraZeneca research award
Introduction: Our goal was to study the hormonal regulation of immune cell infiltration in prostate cancer patients treated by androgen deprivation therapy (ADT) using an optimized computer-assistance quantification approach. Methods: The relative density of immune cell subtypes (CD3(+), CD8(+), CD20(+), CD56(+), CD68(+) and Foxp3(+)) was analyzed by immunohistochemistry in archived prostate specimens from control patients (radical prostatectomy only, n = 40) and ADT-treated patients (ADT prior to radical prostatectomy, n = 35) using an image analysis software and a whole-slide scanner. Results: ADT-treated patients had significantly increased relative density of CD3(+) (p<0.001) and CD8(+) T lymphocytes (p<0.001) as well as CD68(+) macrophages (p<0.001). Elevated abundance of CD56(+) Natural Killer (NK) cells was associated with a lower risk of prostate cancer progression (p = 0.044), while a high density of CD68(+) macrophages was related to an increased risk of biochemical recurrence (p = 0.011). Conclusions: Our results demonstrate that the infiltration of specific immune cell subtypes is modulated by ADT. Furthermore our data confirm that NK cells have a protective role against tumor progression while macrophages seem to favor the development of advanced prostate cancer. (C) 2009 Elsevier B.V. All rights reserved.
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