期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 343, 期 2, 页码 65-67出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2008.11.012
关键词
HIV; Memory B cells; gp140
资金
- Intramural NIH HHS [Z99 AI999999] Funding Source: Medline
- NIAID NIH HHS [R01 AI037526, R01 AI037526-15] Funding Source: Medline
Antibodies to HIV are potentially important reagents for basic and clinical studies. Historically, these reagents have been produced by random cloning of heavy and light chains in phage display libraries [Burton, D.R., Barbas, C.F. III, Persson, M.A.A., Koenig, S., Chanock, R.M., and Lerner, R.A., (1991), A large array of human monoclonal antibodies to type 1 immnodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals. Proc. Nail. Acad. Sci. U. S. A. 88, 10134-10137.] and electrofusion techniques [Buchacher, A., Predl, R., Tauer, C.. Purtscher, M., Gruber, G., Heider, R., Steindl, F., Trkola, A., Jungbauer, A., and Katinger, H., (1992), Human monoclonal antibodies against gp41 and gp120 as potential agent for passive immunization. Vaccines 92, 191-195]. Here we describe a method to identify and potentially enrich human memory B cells from HIV infected patients that show serum titers of neutralizing antibodies. When biotinylated gp140 is used to stain peripheral blood mononuclear cells it identifies a distinct population of gp140 binding B cells by flow cytometry. (C) 2008 Elsevier B.V. All rights reserved.
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