期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 341, 期 1-2, 页码 135-145出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2008.11.009
关键词
Antibodies; Hybridomas; Specificity; Multiplexed; Single cell assay
资金
- SBIR [2R44GM070021-02A7]
The secreted immunoglobulin footprint of single hybridoma cells, containing similar to 10 fg of antibody purified in situ, has been probed for 9 properties concurrently by use of detection labels comprising 280 nm combinatorially colored fluorescent latex beads functionalized with proteins. Specificity of each individual hybridoma cell's product has thereby been assessed in a primary screen. Varying the density of antigen on beads to modulate the avidity of the interaction between bead and secreted antibody footprint allowed rank ordering by affinity in the same primary screen. As more criteria were added to the selection process, the frequency of positive cells went down; in some cases, the favorable cell was present at < 1/50,000. Recovery of the cell of interest was accomplished by plating the cells in a viscous medium on top of a membrane. After collecting the antibody footprint on a capture surface beneath the membrane, the immobilized cells were transferred to an incubator while the footprints were analyzed to locate the hybridoma cells of interest. The desired cells were then cloned by picking them from the corresponding locations on the membrane. (C) 2008 Elsevier B.V. All rights reserved.
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