期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 340, 期 2, 页码 138-143出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2008.10.011
关键词
Bisphenol A; Antibodies; Cationized BSA; Mannich reaction
资金
- National High Technology Research and Development Program of China [2007AA06Z419]
- Natural Science Foundation of China [30771810]
- Ministry of Science & Technology of China [2006BAK02A09]
To detect BPA residue in the environment quickly by immune method, preparation of high quality antibody against BPA was the most critical step of project. Based on Mannich-type reaction and EDC method, we produced BPA-cBSA and BHPVA-BSA against BPA and the conjugates were characterized by UV-Vis and FT-IR spectroscopy. Compared with BSA, cBSA as carrier protein could improve the efficiency of coupling and significantly enhance the immune response against BPA. The mole coupling-ratio of BPA-cBSA (8:1) was greater than that of BHPVA-BSA (5:1). We also observed that the similar sensitivity of antisera against BPA between the BPA-cBSA group and BHPVA-BSA group. (C) 2008 Elsevier B.V. All rights reserved.
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