期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 346, 期 1-2, 页码 38-44出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2009.05.003
关键词
NKT cells; aAPC; CD1d
资金
- NCI NIH HHS [R01 CA108835, R01 CA108835-05, K01 CA131487-01, K01 CA131487] Funding Source: Medline
- NIAID NIH HHS [T32 AI007247-24, R01 AI029575-16, R01 AI029575, R01 AI044129, T32 AI007247, R01 AI044129-03] Funding Source: Medline
Natural killer T (NKT) cells play a pivotal role in maintaining immune homostasis. They recognize lipid antigen in the context of CD1d molecules and subsequently produce cytokines that activate cells of both the innate and adaptive immune responses. Many studies examining patients with autoimmune disease or cancer have shown that there is a reduction in both NKT cell number and function. Due to the complexities of manipulating NKT cells in vivo, ex vivo expanded effector NKT cells would be an excellent therapeutic modality. To date, immunotherapy utilizing the NKT/CD1d system has been dependent on the use of autologous DC in the presence or absence of a synthetic glycolipid, alpha-galactocylceramide. Here we report a novel technique that facilitates the growth and analysis of NKT cells through the use of CD1d-expressing aAPC. CD1d-based aAPC can effectively propagate both canonical (iNKT cells) and noncanonical (V alpha 14(-)) NKT cells. Importantly, CD1d-Ig aAPC can expand NKT cells from cancer patients. Thus, CD1d-expressing aAPC will enhance our knowledge of NKT cell biology and could potentially be used as a novel tool in adoptive immunotherapeutic strategies. (c) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据