期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 340, 期 1, 页码 42-47出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2008.09.025
关键词
Antibody secreting cells; Plasmablasts; Vaccine; Influenza
资金
- NCRR NIH HHS [M01 RR000044] Funding Source: Medline
- NIAID NIH HHS [L30 AI057621, R37 AI049660, R01 AI045969, N01AI50029, N01AI50020, R01 AI084808, R24 AI054953, K23 AI067501, U01 AI045969, U19 AI056390] Funding Source: Medline
- NATIONAL CENTER FOR RESEARCH RESOURCES [M01RR000044] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [U19AI056390, U01AI045969, K23AI067501, R24AI054953, R01AI045969, R37AI049660, N01AI050029, R01AI084808] Funding Source: NIH RePORTER
The rise in influenza-specific neutralizing antibody levels is proceeded by a burst of antigen-specific antibody secreting cells (ASC) or plasmablasts identified in peripheral blood approximately 5-10 days post immunization. Blood antigen-specific ASC may function as an immune marker of vaccine responses in comparison to the pre- and post-neutralizing titers; however, some have questioned whether there is adequate survival of ASC isolated from peripheral blood after freezing, making multi-center vaccine trials difficult. Here, we demonstrate similar frequencies of influenza-specific ASC from fresh and frozen peripheral blood mononuclear cells (PBMC). Influenza Hemagglutinin (HA) H1, H3, and H7-specific ASC IgG ELISpots frequencies were compared from the same fresh and frozen PBMC 7 days after 2006 Trivalent Influenza Vaccine (TIV) in 10 young healthy subjects. H1-, H3-, and H17-specific IgG ASC spots/10(6) from fresh PBMC on day 7 were 229 +/- 341, 98 +/- 90, and 6 +/- 11 respectively. Total IgG ASC spots/million PBMC pre- and 7-day post-vaccination were 290 +/- 188 (0.029% PBMC) and 1691 +/- 836 (0.17% PBMC) respectively. There was no difference in the H1 -H3-, and total specific ASC IgG ELISpot frequencies from the fresh versus frozen PBMC on day 7 (p=0.43, 0.28, 0.28 respectively). These results demonstrate feasibility of testing whether antigen-specific ASC from frozen PBMC are an early biomarker of long-term antibody responses in multi-center vaccine trials. (c) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据