期刊
JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY
卷 30, 期 3, 页码 245-261出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15321810903084350
关键词
Antigenicity; Hemagglutination inhibition; Immunoassay; Influenza; Mass spectrometry
资金
- Australian Research Council [DP0770619]
- Major National Research Facility (MNRF)
- University of Sydney
The antigenicity of a type A (H1N1) influenza strain has been characterised through the application of mass spectrometry (MS) and hemagglutination-inhibition immunoassays performed in parallel. Two monoclonal antibodies were found to be highly and equally specific in HI assays against influenza strain A/New Caledonia/20/99 while the MS immunoassay demonstrated that both antibodies recognise the same epitopic peptide localised to residues 225-232 of the hemagglutinin HA1 subunit whose C-terminal residues reside in close proximity to the receptor binding site. Both immunoassays showed no binding of a monoclonal antibody that recognizes the hemagglutinin antigen of type B strains.
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