期刊
JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES
卷 33, 期 5, 页码 666-671出版社
SPRINGER
DOI: 10.1007/s11596-013-1178-8
关键词
curcumin; low-density lipoprotein; reactive oxygen species; cyclooxygenase-2; p38 mitogen activated protein kinase
资金
- National Natural Science Foundation of China [81100485]
- Scientific Research Foundation for Returned Scholars, Ministry of Education of China [[2011]1568]
Curcumin, as a main pharmacological component in the traditional Chinese medicine-turmeric, has shown anti-inflammatory, anti-oxidation, anti-tumor and anti-fibrotic effects. This study aimed to investigate the possible underlying signaling pathway which was involved in the inhibition of LDL-induced proliferation of mesangial cells and matrix by curcumin. Rat mesangial cells in vitro were incubated with low-density lipoprotein (LDL) and different concentrations of curcumin (0, 6.25, 12.5, 25.0 mu mol/L) or p38 MAPK inhibitor, SB203580 (10 mu mol/L). Under LDL incubation, mesangial cells proliferated, the expression of MMP-2 mRNA and protein was decreased, the expression of COX-2 mRNA and protein was increased, reactive oxygen species (ROS) generation was increased and p38 MAPK was activated significantly (P < 0.05). When LDL-induced cells were treated with curcumin in the concentration of 12.5 or 25.0 mu mol/L, LDL-induced proliferation of mesangial cells was suppressed, the expression of MMP-2 mRNA and protein increased, the expression of COX-2 mRNA and protein downregulated, the production of ROS inhibited and p38 MAPK inactivated (P < 0.05). In conclusion, curcumin can inhibit the LDL-induced proliferation of mesangial cells and up-regulate the expression of MMP-2, which may be related with the inhibitory effect of curcumin on COX-2 expression, ROS production and p38 MAPK.
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