期刊
JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES
卷 31, 期 2, 页码 215-219出版社
SPRINGER
DOI: 10.1007/s11596-011-0255-0
关键词
dracorhodin perchlorate; prostate cancer; proliferation; apoptosis
资金
- Key Project of Science and Technology of Wuhan [201060938361-08]
The growth inhibition and pro-apoptosis effects of dracorhodin perchlorate on human prostate cancer PC-3 cell line were examined. After administration of 10-80 mu mol/L dracorhodin perchlorate for 12-48 h, cell viability of PC-3 cells was measured by MTT colorimetry. Cell proliferation ability was detected by colony formation assay. Cellular apoptosis was inspected by acridine orange-ethidium bromide fluorescent staining, Hoechst 33258 fluorescent staining, and flow cytometry (FCM) with annexin.-FITC/propidium iodide dual staining. The results showed that dracorhodin perchlorate inhibited the growth of PC-3 in a dose-and time-dependent manner. IC50 of dracorhodin perchlorate on PC-3 cells at 24 h was 40.18 mu mol/L. Cell clone formation rate was decreased by 86% after treatment with 20 mu mol/L of dracorhodin perchlorate. Some cells presented the characteristic apoptotic changes. The cellular apoptotic rates induced by 10-40 mu mol/L dracorhodin perchlorate for 24 h were 8.43% to 47.71% respectively. It was concluded that dracorhodin perchlorate significantly inhibited the growth of PC-3 cells by suppressing proliferation and inducing apoptosis of the cells.
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