期刊
ORGANIC & BIOMOLECULAR CHEMISTRY
卷 13, 期 10, 页码 2918-2926出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ob02178a
关键词
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资金
- National Natural Science Foundation of China [21301011]
- State Key Laboratory of Fine Chemicals [KF1305]
- Program for Liaoning Excellent Talents in University [LR2014009]
- Macquarie University Research Fellowship Scheme [MQRF-1487520]
Chemosensors play important roles in cation and anion recognition in biological, industrial, and environmental processes. Although many efforts have been made to develop artificial fluorescent receptors for Cu2+ and S2-, their applications in the detection in bulk solutions are limited. In this work, we report a novel fluorescence chemosensor ( NL) based on the 7-nitrobenz-2-oxa-1,3-diazole ( NBD) fluorophore for the quantification of Cu2+ and S2- in single intact cells. NL specifically binds to Cu2+ in the presence of other competing cations, and evident changes in UV-vis and fluorescence spectra in HEPES buffer are noticed. Based on the displacement approach, the selective sense S2- with the in situ generated NL-Cu2+ ensemble gives a remarkable recovery of fluorescence and UV-vis absorption spectra. The detection limits of NL for Cu2+ and NL-Cu2+ for S2- were estimated to be 1.6 nM and 0.17 mu M, respectively. NL and the resultant complex NL-Cu2+ exhibit low cytotoxicity and cell-membrane permeability, which makes them capable of Cu2+ and S2- imaging and quantification in living MDA-MB-231 cells.
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